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Biology µGCSE:

Cloning + Tissue Culture

10 quick questions - for GCSE and iGCSE

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10 minutes maximum! (can you do it in 5?)

Q1-3: Different varieties of Morning glory plants produce flowers of different colours. Two different varieties are shown in the photograph.

 

morning glory flowers
PiccoloNamek | CC 3.0
1. A gardener likes the purple variety of the morning glory plant and takes 6 cuttings from one plant. The cuttings grows into 6 new plants.

This is an example of ..

  • A. cloning
  • B. selective breeding
  • C. genetic modification
  • D. sexual reproduction

2. Which of the following statements is incorrect?

The new plants will have ..

  • A. the same number of chromosomes as the parent plant
  • B. the same alleles as the parent plant
  • C. only purple flowers
  • D. mixture of purple and pink flowers.

3. Which of the following is not an advantage of cloning plants?

  • A. It can be done at any time of the year
  • B. Large numbers of plants produced
  • C. Lots of different varieties of the parent plant are produced
  • D. All plants are identical

Q4-6: Dolly was the first successfully cloned mammal.
The diagram below shows the cloning process.

Dolly cloned sheep diagram

4. Dolly’s genome comes from ...
  • A. scottish Blackface sheep
  • B. female Finn-Dorset sheep
  • C. male Finn-Dorset sheep
  • D. surrogate ewe.
5. An enucleated cell is a cell that ...
  • A. has a haploid nucleus
  • B. has a diploid nucleus
  • C. has a normal nucleus
  • D. has no nucleus
6. What does the term transgenic mean?
  • A. Transfer of material from one species to a different species
  • B. Production of genetically identical organisms by asexual reproduction
  • C. Mating two individuals of different sexes
  • D. Changing sex from a male to a female.

Q7-10: Tissue culture or micropropagation of plant tissue can be used to propagate plants. Often these plants may have been genetically engineered to include a beneficial gene.

The image shows one of the stages of micropropagation.

 

Dolly cloned sheep diagram
Syed Sajidul Islam | CC 4.0
7. The stages of tissue culture include:
  1. Growth of explants under sterile conditions
  2. Tissue culture plantlets grown in controlled conditions before planting outside
  3. Removal of explant and explant sterilised with bleach
  4. Division of callus to produce many new plantlets

The correct order of the stages is

  • A. 1,2,3,4
  • B. 3,4,1,4
  • C. 2,3,4,1
  • D. 3,1,4,2

8. The explants are grown on a medium that contains nutrients and growth regulators, as shown in this table:

Contents Reason
glucose Respiration and formation of cellulose
Plant hormones Control growth and division of cells
nitrates For the production of proteins
Magnesium ions ?

Why are magnesium ions included?

  • A. Stop bacteria growing
  • B. Help plantlets can make chlorophyll
  • C. Help plantlets respire
  • D. Help plantlets make proteins
9. Which of the following is needed for a high rate of growth of the explants?
  • A. Air with 100% oxygen and warm conditions
  • B. High light intensity and warm conditions
  • C. Cold temperature and high light intensity
  • D. Low light intensity and warm conditions
10. Which of the following is NOT an advantage of tissue culture?
  • A. Used to preserve rare plant species
  • B. Produce large numbers of genetically identical plants with desirable characteristics
  • C. Reproduction of plants is possible without having to wait for the onset of seed production.
  • D. All plants produced will be susceptible to the same disease